Table 2.
Primers used in this study*.
| Name | Position | Sequence | Use |
| SA-hfq 5up (BamH1) | F | 5'CGGGATCCATGACGATTCTTTAGGTGA3' | Used for cloning upstream region of hfq |
| SA-hfq 5down (KpnI) | R | 5'GGGGTACCTGTCGGACTCCTTTTACT3' | |
| SA-hfq 3up (SmaI) | F | 5'TCCCCCGGGATGGGCACGATTTAATGAC3' | Used for cloning downstream region of hfq |
| SA-hfq 3down (EcoRI) | R | 5'CGGAATTCTACTTGAAACACCTGATT | |
| SA-cat 5 (KpnI) | F | 5'GGGGTACCGCACAGACAGGACAAAATCG | Used for cat amplification |
| SA-cat 3 (SmaI) | R | 5'TCCCCCGGGTTCCGAGGCTCAACGTC3' | |
| SA-hfq verif up | F | 5'ATCATAGCAGGTGGAACAG3' | Used for verification of hfq deletion |
| SA-hfq verif down | R | 5'CGAAAGAGAAATAGAAAAAT3' | |
| SA-RNAIII 5 | F | 5'CAGAGATGTGATGGAAAATAGTTG3' | Used for RNA III probe amplification |
| SA-RNAIII 3 | R | 5'ATTAAGGGAATGTTTTACAGTTATT3' | |
| SA-spa 5 | F | 5'TTAGCATCTGCATGGTTTGC3' | Used for spa probe amplification |
| SA-spa 3 | R | 5'ACAACGTAACGGCTTCATCC3' | |
| SA-hu 5 | F | 5'AGATTTAATCAATGCAGTTGCAGA3' | Used for hu probe amplification |
| SA-hu 3 | R | 5'AATGCTTTACCAGCTTTG AATGCT3' | |
| SA-hfq 5 (NdeI) | F | 5'GGAATTCCATATGATTGCAAACGAAAAC3' | Used for hfq probe amplification, RT-PCR |
| SA-hfq 3 (HindIII) | R | 5'CCCAAGCTTATTCTTCACTTTCAGTAG3' |
*F: forward; R: reverse. Restriction enzyme sites are underlined.