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. Author manuscript; available in PMC: 2008 Feb 15.

Published in final edited form as: Biochem Pharmacol. 2006 Oct 27;73(4):469–480. doi: 10.1016/j.bcp.2006.10.023

Fig. 3 — Irofulven induces chromosome aberrations and inhibits DNA synthesis. A, A2780 and CAOV3 cells were treated with 1xIC50 concentrations of irofulven (0.8 and 0.9 μM, respectively) for one hour followed by 24 hours of drug-free incubations. Metaphase spread staining was performed. Arrows indicate chromosome breaks, triradials and quadriradials. B, A2780 and CAOV3 cells were treated with 1xIC50 concentrations of irofulven for one to nine hours. DNA synthesis rate was assessed by BrdU incorporation and FACS analysis. The mean and standard deviation of triplicate experiments were shown.

Fig. 3 — Irofulven induces chromosome aberrations and inhibits DNA synthesis. A, A2780 and CAOV3 cells were treated with 1xIC50 concentrations of irofulven (0.8 and 0.9 μM, respectively) for one hour followed by 24 hours of drug-free incubations. Metaphase spread staining was performed. Arrows indicate chromosome breaks, triradials and quadriradials. B, A2780 and CAOV3 cells were treated with 1xIC50 concentrations of irofulven for one to nine hours. DNA synthesis rate was assessed by BrdU incorporation and FACS analysis. The mean and standard deviation of triplicate experiments were shown.