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. Author manuscript; available in PMC: 2008 Feb 15.
Published in final edited form as: Biochem Pharmacol. 2006 Oct 27;73(4):469–480. doi: 10.1016/j.bcp.2006.10.023

Fig. 4.

Fig. 4

Fig. 4

Fig. 4

CHK2 activation induced by irofulven is DNA replication-dependent. CAOV3 cells were pretreated with aphidicolin (APH, 1 μM, 2 h) before being treated with 0.9 μM of irofulven for one hour followed by 12 hours of incubation in medium containing APH. A, CHK2 activation was determined by Western blot analysis with antibody recognizing Thr 68-phosphorylated CHK2. Blots for CHK2 served as the loading control. B and C, CHK2 foci formation was assessed by immunofluorescent staining. Cells with five or more foci were counted as positive for staining. The percentage of cells with phosphorylated CHK2 foci was exhibited as the mean and standard deviation of triplicate counts of 100 cells.