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Mad2 protein expression in bone marrow cells of Mad2+/− and Mad2+/+ mice. Lineage-depleted (lin−) and -positive (lin+) cells from Mad2+/+ and Mad2+/− mice BM were obtained by magnetic separation. Total-cell lysates were dissolved in SDS-PAGE. Membrane was immunoblotted with anti-Mad2 and antiactin antibodies. +/+ indicates Mad2+/+; +/−, Mad2+/−.
