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. 1999 Sep 28;96(20):11217–11222. doi: 10.1073/pnas.96.20.11217

Figure 1.

Figure 1

Transient activation of the cyclin D1 promoter by estradiol in MCF-7 cells. (A) MCF-7 cells were transfected by using the calcium phosphate procedure with 5 μg of D1–944 Luc and 1 μg of pCH110, then treated with 5 nM ICI 182,780 for 48 h in the presence of 5% charcoal-stripped serum (CSS). At t = 0 h, medium was changed to 5% CSS with 10 nM estradiol. At the times indicated, cells were harvested and assayed for luciferase and β-galactosidase activities. (B) Whole-cell extracts were prepared from a parallel set of transfected cells and analyzed for cyclin D1 protein by Western blotting.