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HbN is expressed in resting cells of M. bovis BCG. M. bovis BCG cells (ATCC 35734) were grown at 37°C with constant shaking. Aliquots of suspension were taken at the indicated times for A580 determination and Western blot analysis of total proteins (arrows). The detection was performed by using polyclonal Abs raised against HbN. Each lane contains 5 μg of proteins.
