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Equilibrium denaturation of gelsolin domain 2. Lines represent the fit of the data to a two-state equation with sloping baselines. (A) Urea titration of wild-type gelsolin 151–266 at 25°C; (B) urea titration of D187N mutant gelsolin 151–266 at 25°C; (C) urea titration of D187Y mutant gelsolin 151–266 at 15°C; and (D) urea titration of wild-type gelsolin 151–266 at 15°C.
