Table 3.

Values obtained from stopped-flow fluorescence studies on gelsolin domain 2

Protein	T, °C	[urea]50%, M	m*, kcal·mol−1 M−1	ΔGkinH2O†, kcal·mol−1
Wild type	25	2.65	1.78 ± 0.10	3.67 ± 0.16
D187N	25	1.85	2.02 ± 0.09	2.43 ± 0.19
D187Y	15	1.82	1.80 ± 0.01	2.62 ± 0.19

^*Calculated from m_kin = m_‡-N − m_D-‡ = RT(m_ku − m_kf). 

^†Calculated from ΔG_kin^H2O = −RT ln [k_u^H2O/k_f^H2O].