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Immunolocalization of protease protein in live eggs by confocal microscopy. Fluorescence labeling of ovotryptase1 (A and B), ovotryptase2 (C and D), and ovochymase (E and F) in unactivated dejellied eggs (A, C, and E) and activated dejellied eggs (B, D, and F). (A–F, bar = 10 μm.) High-magnification phase-contrast image of unactivated eggs (G) and the corresponding fluorescence image (H) show ovochymase labeling. (G and H, bar = 1 μm.)
