Fig. 3.

Effects of α-tubulin polyglutamylation on binding of MAPs to microtubules. (A) Crude microtubules were prepared from brain homogenates of wild-type (+/+) or ROSA22 mutant (−/−) in the presence of ATP or AMP-PNP. Cosedimented kinesins and MAPs were detected by Western blot analysis. The amount of protein applied was monitored by the intensity of the CBB-stained tubulin. (B) Signal intensities were quantified and are represented as a percentage of mutant signal relative to wild-type signal. (Top) ATP-present sample. NA, not applicable. (Middle) AMP-PNP-present sample. (Bottom) Total brain homogenate (bottom). Open columns, wild type; filled columns, mutant. ∗, P < 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.001, with paired t test.