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. 2007 Feb 16;3(4):205–211. doi: 10.7150/ijbs.3.205

Figure 2.

Figure 2

Expression and visualization of the GFP inserted into PI-SceI and VMA1 PI-SceI proteins. A and B, E. coli XL1B (DE3) transformed with pET-28a PI-SceI 117GFP vector. C and D, E. coli XL1B (DE3) transformed with pET-28a vma1 PI-SceI 117GFP3.9kb vector. E and F, E. coli XL1B (DE3) transformed with pET-28a vma1 PI-SceI 117GFP3kb vector. A, C, and E expression was conducted in LB media at 10oC for 72 hours while B, D, and F expression was performed in minimal media at 10oC for 72 hours. Induction and cell grow in minimal media result in expression of soluble protein since the GFP is seen distributed in cytoplasm of the cells. In LB grown cells more expression was detected; however, the expressed proteins, formed inclusion body in parts of cells.