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. Author manuscript; available in PMC: 2007 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2006 Sep 16;1764(11):1741–1749. doi: 10.1016/j.bbapap.2006.09.005

Table 1.

Sedimentation equilibrium results for studies on bovine factor B. According to the variance, number of floated parameters, and monomer molecular weights reported, these results show that the monomer-trimer model is the most appropriate model choice. The 95% confidence intervals determined by Monte Carlo analysis are reported in parentheses.

Equilibrium Model Molecular weight RMS (x 102) Kd Floated Parameters
single ideal species 42.14 kDa (+.38/-.35) 2.380 - 41
monomer-dimer 29.29 kDa (+2.07/-3.31) 1.997 Kd1,2 = 2.57 × 10-5 M (+3.08/-1.40 x 10-5M) 42
monomer-trimer 20.97 kDa (+1.51/-1.08) 1.755 Kd1,3 = 2.48 × 10-10 M2 (+1.42/-0.90 × 10-10 M2) 42
monomer-dimer-trimer 21.04 kDa (+1.44/-1.22) 1.755 Kd1,2: not detectable: Kd1,3 = 2.56 × 10-10 M2 (+1.51/-0.95 × 10-10 M2) 43
Two ideal species,non-interacting 22.61 kDa (+.73/-.68) 60.84 kDa (+2.46/-1.84) 1.595 - 63
Calculated from Protein Sequence 20.42 kDa (monomer)