Figure 5.

Northern blot analysis of RNA replication and transcription. HEp-2 cells infected with rA2-wt (a, d, and g), rA2-NdeI (b, e, and h) and rA2-K5 (c, f, and i) were harvested at 3-hr intervals (lanes 1–10) from the single-cycle growth curve experiment described in Fig. 4A, and total intracellular RNA was isolated and subjected to Northern blot analysis. Blots were hybridized with a negative-sense N-specific riboprobe (a–c), a negative-sense F-specific riboprobe (d–f) or a positive-sense F specific riboprobe (g–i). Monocistronic mRNA, polycistronic readthrough mRNAs and antigenome and genome RNAs are indicated.