Figure 6.

Western blot analysis of the accumulation of F and G glycoproteins in HEp-2 cells infected at an moi of 5 with rA2-wt (A and C) or rA2-NdeI (B and D). Cells were harvested at the indicated time, and total cellular protein was subjected to polyacrylamide gel electrophoresis under denaturing and reducing conditions, transferred to nitrocellulose (5), and reacted with rabbit antipeptide serum against the cytoplasmic domain of the F (A and B) or G (C and D) protein. Bound antibodies were detected with horseradish peroxidase-conjugated goat anti-rabbit IgG and visualized by enhanced chemiluminescence (Amersham Pharmacia).