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. Author manuscript; available in PMC: 2007 Feb 21.
Published in final edited form as: J Biol Chem. 2005 Sep 26;280(49):40757–40765. doi: 10.1074/jbc.M506819200

Fig. 1.

Fig. 1

Phosphorylation of recombinant Knirps protein in Drosophila embryos. FLAG-tagged Knirps protein was expressed in 2–4 hour Drosophila embryos and lysates were analyzed by Western blot. Before heat-shock induction, no specific band is visible (lane 1). After heat shock, a set of bands from 60–80 kDa is visible (arrow, lane 2). These bands collapse to a single species upon incubation of lysates with increasing amount of lambda phosphatase (lanes 3–7), but not in the presence of the phosphatase inhibitor sodium orthovanadate (lanes 8–9). A nonspecific product is marked with the asterisk.