Table 1.

Structural statistics for the Apaf-1 CARD

NOE distance restraints
All	1,219
Intraresidue	645
Sequential (|i − j| = 1)	214
Medium range (|i − j| ≤ 4)	166
i, i + 2	9
i, i + 3	115
i, i + 4	32
Long range (|i − j| ≥ 5)	118
H bonds*	76
Dihedral angle constraints†	322
Ramachandran plot‡
Most favorable region, %	85.8
Additionally allows region, %	14.2
Generously allowed region, %	0.0
Disallowed region, %	0.0
Average RMS deviation to the mean structure
Backbone (residues 3–91) Å	0.39
Heavy atoms (residues 3–91), Å	0.77

None of these structures exhibit distance violations greater than 0.4 Å or dihedral angle violations greater than 5°. 

^*Hydrogen bond for α-helices was added only at the late stage of structural calculations for residues with characteristic HN(i)-Hα (i − 3, i − 4) NOEs observed in the three-dimensional ¹⁵N-NOE spectroscopy-HSQC spectrum. 

^†Dihedral angle constraints were generated by dyana (29), based on ³J_HNHα couplings, Cα chemical shifts (30), and NOE constraints. 

^‡procheck_nmr (35) was used to assess the quality of the structures.