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. 2005 Sep;116(1):71–81. doi: 10.1111/j.1365-2567.2005.02199.x

Figure 1.

Figure 1

FN, DA and EQ enhance IL-4 production and mRNA expression in KLH-stimulated CD4+ T cells. (a) Chemical structures of formononetin and its metabolites. (b) Isolated CD4+ T cells, as described in the Materials and methods, were re-stimulated with KLH (100 µg/ml) in the presence of FN (10, 50 µm), DA (10, 50 µm) and EQ (10, 25 µm) for 4 days. The cell culture supernatants were harvested and assayed for IL-4 and IFN-γ levels by ELISA. (c) Isolated CD4+ T cells were re-stimulated with KLH (100 µg/ml) in the absence or presence of FN (50 µm), DA (50 µm) and EQ (25 µm) for 6 hr. Cellular RNA from each treatment was extracted and the mRNA expression for IL-4, IFN-γ and β-actin was analysed by RT–PCR. The values represent the means ± SE of three independent experiments. *P < 0·05 and **P < 0·005, relative to the corresponding control treated with KLH alone.