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. 2005 Sep;116(1):71–81. doi: 10.1111/j.1365-2567.2005.02199.x

Figure 2.

Figure 2

Enhancement of IL-4 production in activated EL4 cells by formononetin and its metabolites. (a) EL4 cells were stimulated with PMA (1 ng/ml) in the presence of varying amounts of FN, DA and EQ for 2 days. The cell culture supernatants were harvested and assayed for IL-4 levels by ELISA. (b) EL4 cells were stimulated with PMA (1 ng/ml) in the absence or presence of FN, DA and EQ for 6 hr. Cellular RNA from each treatment was extracted and the mRNA expression for IL-4 and β-actin was analysed by RT–PCR. The values represent the means ± SE of three independent experiments. *P < 0·05 and **P < 0·01, relative to the corresponding control treated with PMA alone.