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. 2005 Sep;116(1):71–81. doi: 10.1111/j.1365-2567.2005.02199.x

Figure 3.

Figure 3

FN, DA and EQ enhance IL-4 gene promoter activity induced by PMA. (a) The schematic diagram of the proximal 750 base pairs of murine IL-4 gene promoter. The P and MARE represent NF-AT binding sites and c-Maf binding sites, respectively. P1 and P4 sites include AP-1 binding site. (b) EL4 T cells were transiently transfected with the IL-4 full promoter construct, followed by stimulation with PMA (1 ng/ml) in the absence or presence of FN (10, 50 µm), DA (10, 50 µm) and EQ (10, 25 µm). The results are represented as the induction fold over the value obtained with unstimulated EL4 cells transfected with the promoter construct with given an arbitrary value of 1. (c) EL4 cells were transiently transfected with the serially deleted IL-4 promoter constructs, followed by stimulation with PMA (1 ng/ml) in the absence or presence of FN (50 µm), DA (50 µm) and EQ (25 µm). The values represent the means ± SD of triplicate determinations. The data are representative of three independent experiments. *P < 0·01 and **P < 0·001, compared with each of IL-4 promoter construct treated with PMA alone.