Figure 7.

Involvement of PI3-K, PKC and p38 MAPK in the enhanced AP-1 binding activities by FN, DA and EQ.EL4 cells were treated with 10 µm LY294002 (L), 1 µm GF109203X (G) and 10 µm SB203580 (S) for 40 min, followed by stimulation with PMA (1 ng/ml) in the absence or presence of FN (50 µm), DA (50 µm) and EQ (25 µm). DNA binding activities were assessed by EMSA. In EMSA α-³²P-end labelled DNA probe that included the AP-1 binding domain of murine IL-4 promoter was incubated with nuclear extracts (10 µg/lane). Specificity of binding was determined by the use of a 50-fold excess of specific or unrelated oligonucleotide (nuclear factor-κB). The data are representative of three independent experiments.