Figure 8.
Increased susceptibility to neosporosis in mice immunized with N. caninum antigens. Survival rates of mice infected i.p. with 5 × 106 NcT 30 days after the second i.d. inoculation at a 3-week interval of (a) alum (open circles) or 10 µg of NcS in alum adjuvant (closed triangles), (b) Freund's adjuvant (open circles) or 10 µg of NcS in Freund's adjuvant (closed lozenges), (c) PBS (open circles) or 5 × 105 gamma-irradiated NcT in PBS (closed squares). The survival rate of mice sham-immunized was significantly different from that of immunized mice immunized in all the groups (median survival time in days (MST) = 7 (95% CI: 6; 8) chi-square = 12·1, d.f. = 1, P < 0·0006; MST = 8 (95% CI: 7; 9) chi-square = 11·4, d.f. = 1, P < 0·0008; MST = 9 (95% CI: 8; 10) chi-square = 8·1, d.f. = 1, P < 0·005, for results shown in panels A, B and C, respectively). Six mice per group were used. This is one representative result of two independent experiments.