Figure 9.
Decreased IFN-γ mRNA expression, comparatively to sham-immunized controls, in BALB/c mice immunized with N. caninum antigens and challenged with N. caninum tachyzoites. Levels of IFN-γ mRNA expression, normalized to HPRT mRNA, detected by real time RT–PCR, 6 days after challenge i.p. with 5 × 106N. caninum tachyzoites, in the spleen of mice (a) sham-immunized with alum (open bars) or immunized with NcS in alum adjuvant (closed bars); (b) sham-immunized with Freund's adjuvant (open bars) or immunized with NcS in Freund's adjuvant (closed bars); (c) sham-immunized with PBS (open bars) or immunized with irradiated NcT in PBS (closed bars). Bars represent the mean plus 1 SD of three mice per group. This is one representative result of two independent experiments.