Table 2.

ELISA titres of IgG2a and IgM antibodies anti-N. caninum sonicates (NcS), C. albicans extracellular proteins (CaEP), C. albicans sonicates (CaS), Streptococcus agalactiae extracellular proteins (SaEP) or S. agalactiae sonicates (SaS), detected in BALB/c mice sera collected 15 days after i.p. injection with PBS, 10⁶Leishmania infantum promastigotes, 5 × 10⁵N. caninum tachyzoites (NcT) or with the same number of irradiated NcT (Irr-NcT)

Mice groups	NcS	CaS	CaEP	SaS	SaEP
IgG2a
PBS	ND1	ND	ND	13 ± 152	10 ± 17
L. infantum	70 ± 34**	20 ± 12*	5 ± 7	108 ± 10**	98 ± 42*
NcT	6075 ± 4670**	ND	ND	7 ± 11	32 ± 21
Irr-NcT	2145 ± 1275**	ND	ND	ND	5 ± 6
IgM
PBS	811 ± 46	150 ± 104	396 ± 228	1953 ± 1022	1134 ± 428
L. infantum	864 ± 93	1242 ± 187**	1224 ± 82**	4212 ± 1122*	4724 ± 306**
NcT	3564 ± 1223*	241 ± 51	540 ± 270	3454 ± 1686	1134 ± 290
Irr-NcT	1674 ± 247**	244 ± 96	630 ± 311	2943 ± 1032	1458 ± 730

¹ND, not detected.

²Numbers represent means ± 1SD of three serum samples on the PBS group and of four samples in the L. infantum, NcT or Irr-NcT groups. This is one representative result of three independent experiments. Significance of the results between control (PBS) and NcT or Irr-NcT groups determined using anova is indicated in the table

^*P < 0·05

^**P < 0·01.