Figure 5.

(a) Alignment of the nucleic acid sequence of IgM xenoantibodies from monkeys exposed to solid organ hearts. Three individual representative cDNA clones from each time-point were aligned with the closest human germline gene progenitor (IGHV3-11) and the rhesus germline IGHV3-11 (bold, italic). Amino acid translations of the sequences are shown compared with the sequence of the human IGHV3-11 germline progenitor encoding human xenoantibodies (b). The amino acid sequence of the IgM constant region of Macaca mulatta and Homo sapiens is compared to the sequence we obtained in monkeys exposed to porcine heart xenografts (c) (bold, italic). The sequence from the constant region of Homo sapiens and Macaca mulatta IgM heavy chain is listed in the NCBI GenBank database under accession numbers AY671780 and AF046784.