Figure 3.

Expression of CD25 on ovalbumin (OVA) T-cell receptor (TCR) transgenic (tg) CD4⁺ T cells from the celiac lymph node (CLN) (a, c, e) and mesenteric lymph node (MLN) (b, d, f) of BALB/c recipients, after 3 days (a, b) and 7 days (c, d) of OVA administration and on day 7 for the non-fed control group (e, f). The R3 gate represents all cells stained positive for CD25, while the R4 gate only includes the CD25^bright cells. Data shown are from one representative experiment out of four (a, b, e, f) or six (c, d) independent experiments.