Figure 6.

Rottlerin does not decrease polymerized actin or change the surface topology of monocyte derived dendritic cells (MDDCs). (a) The amount of filamentous actin (F-actin) in MDDCs was determined by staining with fluorescein isothiocyanate (FITC)-phalloidin and determining fluorescence by flow cytometry as described in Materials and methods. Data shown are fluorescence histograms of MDDCs with and without treatment for 15 min with 10 µm rottlerin. (b) Scanning electron micrographs of MDDC treated for 5 min with buffer control or 10 µm rottlerin.