Figure 7.

Rottlerin impairs MDDC spreading. MDDCs were allowed to spread for 30 min at 37° on poly L-lysine-coated coverslips in the presence of graded concentrations of rottlerin (A: none; B: 0·1 µm; C: 0·3 µm; D: 1 µm; E: 3 µm; F: 10 µm); thereafter cells were fixed, permeabilized, stained with alexa 488-phalloidin and examined by fluorescence microscopy.