Figure 6.

In vivo expansion of CD62L⁺ (circles) and CD62L^– (triangles) D^d/p18 tetramer⁺ CD8⁺ T cells following antigen stimulation. A total of 2–8 × 10⁵ CD62L⁺ or CD62L^– D^d/p18 tetramer⁺ CD8⁺ T cells were harvested from spleens of 13–16 Balb/c mice at 5 or 8 months postinoculation with 2 × 10⁷ plaque-forming units (PFU) of recombinant vaccinia virus expressing human immunodeficiency virus (HIV)-1 B10 gp160 (rVac-Env), sorted into CD62L⁺ and CD62L^– CD8⁺ T-cell populations, and adoptively transferred into naïve Balb/c mice. Recipient mice were boosted with 10^6.5 particles of rAdeno-Env intramuscularly (i.m.) at the time of adoptive transfer. Plotted values represent the median ± standard error of two separately performed experiments with n = 5–7 (CD62L⁺) or n = 4–6 (CD62L^–) recipient mice.