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. 2006 Dec 8;35(1):247–255. doi: 10.1093/nar/gkl1022

Figure 2.

Figure 2

Suppression of HIV-1 gene expression occurs at the level of both viral protein and RNA. Cells were transfected with proviral DNA (pHxb2 R-/RI-) and 4 μg of U1 wt/αCAT expression vector or increasing amounts of αHIV #1/#5 (0.25, 0.5, 1, 2 or 4 μg) alone or in combination. Forty-eight hours post-transfection, cells were harvested and either (A) used to examine HIV-1 p24 expression or (B) RNA extracted and levels of RNA determined by northern blot. Representative gels are shown indicating the position of the viral RNAs (upper), GAPDH (middle) and U1 snRNA variants (lower).