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. 2006 Dec 8;35(1):247–255. doi: 10.1093/nar/gkl1022

Figure 4.

Figure 4

SL2mut variants of anti-HIV U1 snRNAs retain anti-viral activity. (A) Cells were transfected with HIV-1 proviral DNA (HIV-1 Hxb2 R-/RI-) and vector expressing anti-HIV #1/#5 SL2mut snRNA either alone or in combination. Forty-eight hours post-transfection, cells were harvested and lysates analyzed for p24 expression by western blot. (B) Cells were transfected with HIV-1 proviral DNA (HIV-1 Hxb2 R-/RI-) and vector expressing anti-HIV #1/#5 wild-type, SL2mut, or SL1mut snRNAs either alone or in combination. Total RNA was subsequently extracted and levels of viral RNA, and GAPDH RNA assayed by northern blot. Shown is representative gel of the results obtained. Identity of viral RNAs, GAPDH RNA and U1 snRNA variants (lower) are as indicated.