Table 1.

Summary of targeted sites in HIV-1 provirus and their effect on viral p24 expression

U1 snRNA name	Targeted sequence	Location	Reading frame	Suppression of p24 expression	Conservationa
1	CTTCAGCTAC	8519	Rev exon 2	++	66%
2	ATAGCAGTAG	8682	Env	−	67%
3	TTTGCTATAA	8786	Env	++	56%
4	CAGGTACCTT	9013	Nef	−	37%
5	CATCCGGAGT	9391	LTR	+	71%
6	TGGGGTACCT	6344	Env	−	N/A
7	GCCTGTGTAC	6441	Env	+	N/A
8	GAAAAGGGGG	9073	Nef	−	92%
9	GACTGGAAGG	9083	LTR	−	56%
10	ACACACAAGG	9143	LTR	−	78%
11	AACTACACAC	9172	LTR	−	74%
12	GGGGGACTGG	9079	LTR	+	95%
13	TTTTAAAAGA	9065	Nef	−	91%
14	GGCAGGGATA	8350	Env	−	85%
15	TTAGGCAGGG	8347	Env	−	87%

Position of targeted sequences is stated relative to the reference HIV-1 provirus HXB2 (K03455).

^a% of time the complete target sequence is found in a database of 1005 viral genomes belonging to the M and O clades of HIV-1.

++-High activity, suppression of viral p24 expression to background level.

+-Moderate activity, >50% reduction in p24 production expression.

−-No significant change in viral p24 expression observed.