Table 2.
Plasmids used in this study
| Plasmid | Description | Reference |
|---|---|---|
| pRS415 | Vector for generating transcriptional fusion to lacZ | (34) |
| pBAD33 | Arabinose-inducible expression vector | (35) |
| pET16b | IPTG-inducible expression vector allowing fusion of a N-terminal His10 tag to a target protein | Novagen |
| pET22b(+) | IPTG-inducible expression vector allowing fusion of a C-terminal His6 tag to a target protein | Novagen |
| pTYB1 | IPTG-inducible expression vector allowing fusion of a bifunctional tag, consisting of an intein and a chitin binding domain, to the C-terminus of a target protein | New England Biolabs |
| pRSyy_wt | yefM-yoeB promoter-operator region cloned as annealed oligonucleotides 1/2 between BamHI and EcoRI restriction sites of pRS415 | This study |
| pRSyy_Smut | yefM-yoeB promoter-operator region with mutagenized S repeat cloned as annealed oligonucleotides 3/4 between BamHI and EcoRI restriction sites of pRS415 | This study |
| pBADyefM | yefM amplified with oligonucleotides 5/6, digested with XhoI–HindII and cloned between the equivalent sites in pBAD33 | This study |
| pBADyefMyoeB | yefM-yoeB amplified with oligonucleotides 5/7, digested with XhoI–HindII and cloned between the equivalent sites in pBAD33 | This study |
| pET16yefM | yefM amplified with oligonucleotides 8/9, digested with NdeI–XhoI and cloned between the equivalent sites in pET16b | This study |
| pET22yefM | yefM amplified with oligonucleotides 8/10, digested with NdeI–XhoI and cloned between the equivalent sites in pET22b(+) | This study |
| pTYByefM | yefM amplified with oligonucleotides 8/11, digested with NdeI–SapI and cloned between the equivalent sites in pTYB1 | This study |
| pET16yefMyoeB | yefM-yoeB amplified with oligonucleotides 8/12, digested with NdeI–XhoI and cloned between the equivalent sites in pET16b | This study |
| pET22yefMyoeB | yefM-yoeB amplified with oligonucleotides 8/13, digested with NdeI–XhoI and cloned between the equivalent sites in pET22b(+) | This study |