Figure 5.

Transcript levels of Abf1-dependent genes at 25°C and after 1 h at 37°C in yeast expressing Abf1(1–592) or the corresponding ts mutant. (A) Growth of yeast expressing Abf1(1–592) or the corresponding ts mutant after 2 days at the indicated temperature on YPD plates. (B) Transcript abundance, measured by northern analysis and normalized to PYK1 mRNA, of TCM1/RPL2, QCR8 and PRO3, from yeast expressing full-length Abf1 (wt) or Abf1(1–592) or the corresponding ts mutants, as indicated, grown at 25°C or for 1 h at 37°C. SDs(n = 2–4) are indicated. Note that relative abundances of transcripts from yeast expressing full-length Abf1 (top) or Abf1(1–592) (lower panel) can be directly compared, as transcript levels were measured on the same blots (see Materials and Methods).