Figure 4.

Regulation of NTL expressions by H₂O₂, MMS and growth hormones. (A) Effects of H₂O₂. Plants were subjected to a vacuum/release cycle in hydroponic MS media supplemented with 50 mM H₂O₂ and subsequently grown under normal growth conditions for the indicated time periods. (B) Effects of MMS. Plants were incubated in hydroponic MS media containing 100 p.p.m. MMS for the indicated time periods. (C) Growth hormone effects. Plants that were grown for 2 weeks on MS-agar plates were incubated in hydroponic MS media supplemented with appropriate growth regulators. They were used at the final concentrations of 100 μM for ABA and indole-3-acetic acid (IAA), 50 μM for 1-aminocyclopropane-1-carboxylic acid (ACC), BA and paclobutrazol (PAC), 20 μM for gibberellic acid (GA) and 1-N-naphthylphthalamic acid (NPA), and 1 μM for brassinolide (BL) and brassinazol (BRZ).