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. 2006 Dec 7;35(1):214–222. doi: 10.1093/nar/gkl1072

Figure 3.

Figure 3

Retrohoming of RmInt1 into the intronless RMO17 genome. Panels show Southern hybridizations of SalI-digested DNA from the RMO17 strain (wt) and derivatives harboring plasmids pKG2.5 (donor of the wild-type intron, left panel), pKGEMA4 (expressing a ΔORF variant, right panel), pKG2.5x or pKGEMA4x (both expressing non-mobile introns). The different lanes correspond to individual clones showing all the homing events, using as donor plasmids either pKG2.5 or pKGEMA4. Probe was specific to the 5′ end of the intron ribozyme sequence. D indicates the hybridization signal corresponding to the donor plasmid DNA. Genomic insertions of the wild-type intron (IS+I) or its ΔORF variant (IS+ΔI) are marked. IS were denoted with numbers (1–13) according to the hybridization pattern provided by SalI-digested total DNA using an IS probe numbering from the larger to the smaller hybridizing IS-containing fragment. Number of transconjugants with the observed events is indicated in brackets alongside the bands.