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. 2006 Dec 7;35(1):214–222. doi: 10.1093/nar/gkl1072

Figure 5.

Figure 5

Mobility of the genomic intron copies and trans complementation of the ΔORF derivative with the IEP. (A) Wild-type RmInt1 and ΔORF profiling in RMO17 derivatives cured from donor plasmids pKG2.5 (RMO17-2.5c2) or pKGEMA4 (RMO17-ΔIc50). Genomic DNA from both strains was SalI-digested and probed with a DNA fragment specific to the 5′ end sequence of the RmInt1 ribozyme. (B) In vivo homing of the acquired genomic introns. Target plasmid pJB0.6LAG was mobilized into RMO17-2.5c2 and RMO17-ΔIc50 strains. Compatible plasmids pKGIEP or pKG4dV expressing a functional IEP were then conjugated into RMO17-ΔIc50 containing pJB0.6LAG to complement the ΔORF insertions. Homing of wild-type RmInt1 and ΔORF derivative was assessed on plasmid pools from RMO17-2.5c2 and RMO17-ΔIc50, respectively, as described. pKGIEPYAHH was used as negative complementation control in the assays. Invasion rates were calculated as described in materials and methods and plotted in the histogram shown below. D, donor plasmid; H, homing product.