Table 3.

Experimental results for Promoter PQS with frequent NHEs

Name	No NHEa	Sequence	Tm (/°C)
GUK1	3	agcGGGAGAAGACGGGCTGGGAGGGcgc	52
MCM2	3	gaaGGGACACGGAGGGGCGGGCCAGAGGGtcc	64
NKFB2	3	tcaGGGTGGGGGCCCCGAGGGCTGGGGccg	69
PSMA2	4	gctGGGTTGGGGCGGGGGGAGCGGGacg	69
UTX	3	gccGGGCGGGGAGGGGGGGtca	>80°Cb

Gene names: GUK1, Guanylate kinase 1; MCM2, DNA replication licensing factor; NKFB2, DNA-binding factor KBF2, Lyt10; PSMA2, Proteasome subunit alpha type 2; UTX, Ubiquitously transcribed X chromosome tetratricopeptide repeat protein.

^aNumber of NHE cleavage positions reported within 50 bp of the PQS.

^bThis sequence was further studied in 20 mM KCl and showed a complete melt with a T_m of 66°C.