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. 2006 Dec 14;35(2):414–423. doi: 10.1093/nar/gkl1060

Figure 5.

Figure 5

Expression of wild-type fowlpox virus GPx4 in mammalian cell lines. (A) Western blot analysis of mouse hepatoma cell line Hepa1-6 transfected with wild-type fowlpox virus GPx4 construct (WT FPV GPx) or the corresponding construct in which the SBP2-binding site was disrupted (WT FPV GPx ΔATGA). (B) Transfection of HEK293 with wild-type fowlpox virus GPx4 (FPV GPx), its mutants lacking SBP2-binding site (WT FPV GPx ΔATGA) or the conserved AA in the apical loop (WT FPV GPx ΔAA), and the corresponding Sec-to-Cys mutants.