Figure 2.

Overlay of the ¹H¹⁵N TROSY-HSQC spectra (600 MHz) of L11 in its free form with the RNA bound form (A) and of L11 in the RNA bound form with the RNA and thiostrepton bound form (B). The backbone assignments for L11 are shown for its free form (A) and RNA bound form (B) and some of the major peak shifts are indicated by arrows. The spectra were aquired at 298 K in the same buffer solution [20 mM potassium phosphate buffer (pH 6.1), 200 mM KCl and 5% D₂O].