Figure 5.
Internal dynamics of L11. Heteronuclear relaxation rates ({1H}-15N HetNOE, T1 and T2), T1/T2 ratios and the order parameters (S2) of the backbone amides of L11 in its free form (blue), in complex with RNA (red) and in complex with RNA and thiostrepton (green). Missing bars indicate prolines or amide resonances that have not been (unambiguously) assigned or relaxation values that could not be determined reliable. The order parameter is indicated on the surface of the structures on the right [from blue (rigid, >0.9) to red (flexible, <0.55)]. The experiments were performed at 298 K in the same buffer solution [20 mM potassium phosphate buffer (pH 6.1), 200 mM KCl, 5% D2O) at a field strength of 14.1 Tesla (600 MHz)].