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. 2006 Dec 14;35(2):441–454. doi: 10.1093/nar/gkl1066

Figure 6.

Figure 6

L11 domain orientations as determined using RDCs. Overlay of L11 structures calculated for the protein in its free form (blue), in complex with RNA (red) and in complex with RNA and thiostrepton (green). Every orientation is presented by a bundle of the 20 best structures shown as backbone trace. The free form L11 structure was calculated using NOE and RDC data as shown before (36). The structures for L11 in complex with RNA or RNA and thiostrepton were calculated by SA using Cα distance restraints from different X-ray L11 structures and measured RDCs. The structures are aligned on the stable secondary structure elements of L11ctd (residues 76–83, 98–100, 102–112, 121–132 and 137–139). The figures on the upper panel (A) include the van der Waals surface representation for the RNA that has been included by alignment of the X-ray structure of the L11–RNA complex (26). The figures on the lower panel (B) show a different orientation of the bundle and the calculated principal axis of the mass tensor.