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. 2006 Dec 15;35(2):606–613. doi: 10.1093/nar/gkl1087

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© 2006 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Number of predicted exonic splicing enhancers motifs do not correlate with splicing efficiency. The percentages of exon inclusion detected by splicing assay were compared with the number of predicted exonic splicing regulatory motifs. The analysis includes 22 minigenes with a single substitution, 6 with multiple synonymous substitutions (shown in Figure 4) and 49 minigenes derived from random multiple synonymous substitutions (shown in Figure 2). Splicing regulatory motifs were predicted using RESCUE-ESE (3), ESE finder (19) and PEXS (26) programs.