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. 2006 Dec 7;35(2):e9. doi: 10.1093/nar/gkl979

Figure 2.

Figure 2

Growth curves, sensitivity to DNA-damaging agents and chromosomal aberrations induced by X-ray in RAD18−/− cells. (A) Growth curves of wild-type (closed circles) and RAD18−/− cells (open circles). The data show typical experimental results of three independent experiments. (B) Sensitivities of wild-type (closed circles) and RAD18−/− cells (open circles) to continuous treatment with cisplatin or MMS. Sensitivities to UV light or X-ray irradiation are also shown. The data shown are means of three independent experiments with standard deviations (error bars). (C) Chromosomal aberrations in HCT116 and RAD18−/− cells after X-ray irradiation. Cells were irradiated with 1.4 Gy of X-rays and cultured thereafter. Cells were treated with 0.05 μg/ml colcemid for 1 h prior to harvest and chromosomes were prepared as described in Materials and Methods. A total of 100 metaphase cells were examined in each sample. Chromatid-type (upper) and chromosome-type (lower) aberrations in HCT116 (open circles) and RAD18−/− (closed circles) cells were counted. Approximate cell cycle phases (determined by separate experiments) at the time of X-ray irradiation are shown at the top of the figure. The number of spontaneous chromatid-type aberrations in HCT116 and RAD18−/− cells were 0.06/cell and 0.06/cell, respectively. The number of spontaneous chromosome-type aberrations in HCT116 and RAD18−/− cells were 0.09/cell and 0.07/cell, respectively.