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. 2006 Dec 7;35(2):e9. doi: 10.1093/nar/gkl979

Figure 4.

Figure 4

Monoubiquitination of PCNA. (A) UV light- or HU-induced monoubiquitination of PCNA as determined by western blot analysis. Wild-type HCT116 or RAD18−/− cells were irradiated with 20 J/m2 of UV light or cultured in the presence of 1 mM HU and harvested at indicated times. (B) Monoubiquitination of PCNA after treatment with UV light, X-ray, VP-16 or CPT. HCT116 or RAD18−/− cells were irradiated with UV light (20 J/m2) or X-rays (5 Gy) and harvested after 5 or 24 h incubation for western blot analysis. Cells were cultured in the medium containing CPT (5 or 25 nM) or VP-16 (0.6 or 3 μM) and harvested at indicated times.