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. 2006 Dec 7;35(2):e9. doi: 10.1093/nar/gkl979

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© 2006 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Restoration of CPT sensitivity and UV-induced PCNA monoubiquitination in RAD18−/− cells expressing FLAG-tagged human RAD18. (A) Detection of FLAG-tagged RAD18 using anti-FLAG antibody or anti-human RAD18 antibody. Seven hygromycin-resistant clones (clones 5, 15, 28, 38, 41, 44 and 45) were examined for the expression of the FLAG-tagged RAD18 by western blot analysis. (B) Restoration of UV light-induced monoubiquitination of PCNA in clones (clones 28 and 41) expressing FLAG-tagged RAD18, but not in non-expressing clones (clones 5 and 38). (C) Restoration of CPT sensitivity in clones expressing FLAG-tagged RAD18 (clones 28 and 48), but not in non-expressing clones (clones 5 and 38) as determined by colony-forming assay.