Table 2.

Frequency of stable transformation

Expt no.	Cells	Plasmid DNA (bC or cL)	No. of cells plated	Plating efficiency	No. of cells tested	No. of hygror clones	aS.T. frequency
1	HCT116	pTK-hygro (C)	2 × 106	0.63	1.27 × 106	8	6.3 × 10−6
	HCT116	pTK-hygro (L)	2 × 106	0.64	1.28 × 106	11	8.6 × 10−6
	RAD18−/−	pTK-hygro (C)	2 × 106	0.51	1.01 × 106	39	38.6 × 10−6
	RAD18−/−	pTK-hygro (L)	2 × 106	0.43	0.86 × 106	42	49.0 × 10−6
2	HCT116	pTK-hygro (C)	2 × 106	0.60	1.20 × 106	10	8.3 × 10−6
	HCT116	pTK-hygro (L)	2 × 106	0.74	1.47 × 106	10	6.8 × 10−6
	RAD18−/−	pTK-hygro (C)	2 × 106	0.41	0.82 × 106	74	90.6 × 10−6
	RAD18−/−	pTK-hygro (L)	2 × 106	0.43	0.85 × 106	70	82.4 × 10−6

^aST, stable transformation; ^bC, circular; ^cL, linear.