N. gonorrhoeae strains |
F62 |
Parental strain |
Laboratory stocks |
RUG7001 |
F62 proAB paniA::lacZ
|
[43] |
RUG7022 |
F62 proAB paniA::lacZ fnr
|
[6] |
JCGC201 |
F62 proAB pccp::lacZ
|
[5] |
JCGC202 |
F62 proAB pccp::lacZ fnr
|
[5] |
JCGC502 |
F62 fnr-3xFLAG KanR
|
This work |
E. coli strains |
RV |
Parental strain |
Laboratory stocks |
JCB386 |
Δ (nirB-cycG) pnirB::lacZ
|
[44] |
JCB3861 |
JCB386 narXL
|
This work |
JCB3862 |
JCB386 narXL narQ
|
This work |
JCB3863 |
JCB386 narXL narQ narP
|
This work |
JCB391 |
RV narXL narQ pcnB
|
This work |
JCB12 |
RV narX narQ frdA::lacZ
|
[10] |
Plasmids |
pGCFNR3 |
Gonococcal fnr, under the regulation of the E. coli fnr promoter, cloned into pBR322. |
[5] |
pGCFNRi |
pGCFNR3 with KpnI and XhoI restriction sites engineered downstream of the fnr gene |
This work |
pGCFNR-FLAG |
pGCFNR3 with a 3xFLAG tag and Kanamycin resistance cassette inserted downstream of the fnr gene |
This work |
pSUB11 |
Epitope tagging plasmid carrying 3xFLAG tag and kanamycin resistance cassette. |
[36] |
pGCNarQP |
Gonococcal narQP genes under the control of the E. coli fnr promoter cloned into pBR322. |
This work |
pBADgcQ |
Gonococcal narQ gene under the control of the araBAD promoter cloned into pBAD myc-His A. |
This work |
pBADecQ |
E. coli narQ gene under the control of the araBAD promoter cloned into pBAD myc-His A |
This work |
pRNW18 |
pBADEcQ containing R54K substitution in narQ
|
This work |
pRNW19 |
pBADEcQ containing N48E I49E substitution in narQ
|
This work |
pRNW20 |
pBADEcQ containing N48E I49E R54K substitution in narQ
|
This work |
pRNW29 |
pBADEcQ containing D43A E45S A46V N48E I49E substitution in narQ
|
This work |
pRNW30 |
pBADEcQ containing D43A E45S A46V substitution in narQ
|
This work |
pRNW31 |
pBADEcQ containing D43A E45S A46V R54K substitution in narQ
|
This work |
pRNW32 |
pBADEcQ containing D43A E45S A46V N48E I49E R54K substitution in narQ
|
This work |
pRNW33 |
pBADEcQ containing D43A E45S A46V S52N S57A substitution in narQ
|
This work |
pRNW34 |
pBADEcQ containing D43A E45S A46V N48E I49E S52N R54K S57A substitution in narQ
|
This work |
p7150 |
E. coli nirB promoter cloned into the lacZ reporter vector pRW50. |
[19] |
pRNW15 |
E. coli napF promoter cloned into the lacZ reporter vector pRW50. |
This work |