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. 2007 Feb 8;8:46. doi: 10.1186/1471-2164-8-46

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Copyright © 2007 Anisimov et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Normalization and reproducibility of the NeuroStem Chip arrays. (A-B) Representative plots depict Log₂(ratio) versus log₁₀(intensities) prior to (A) and following (B) the normalization of one technical replicate (hESCs (Cy3) : universal reference (Cy5), 10:5 pmol). Green lines represent fitted values after normalization. (C) Centroid graph of a K-means classifier of 101 genes clustered as highly up-regulated in the 4 technical replicates, indicating high reproducibility. Pink line is an average of the Log2 ratio values of the 101 genes. See [Additional file 1] for a list of genes in this cluster.