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. 2007 Feb 23;3(2):e22. doi: 10.1371/journal.ppat.0030022

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© 2007 Chun et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Proteins extracts from wild-type and two independent cultures of FLAG-SRE1 grown in normoxic and hypoxic (bubbling mixed gas into flasks; 0.2% oxygen) conditions were fractionated by SDS-PAGE and immunoblotted with antibodies against the FLAG epitope. Immunoblotting with antibodies against the cyclin-dependent protein kinase PSTAIRE was used to control for variation in loading.