Table 3. Relative fitnessa (±s.e.m.) of wt and mt virus after 110 days of culture in the C8166 T-cell line.
Virus after 110 days of culture | Cell type-MT4 | Cell type-C8166 | ||||
Fitness | df | Fc | Fitness | df | F | |
d = 110b | d = 110 | |||||
wt-1-C8166 | 1.02±0.01ns | 3 | 2.076ns | 1.01±0.01ns | 9 | 0.530ns |
wt-2-C8166 | 1.01±0.01ns | 3 | 1.753ns | 1.02±0.01ns | 9 | 0.437ns |
wt-3-C8166 | 1.01±0.01ns | 3 | 2.842ns | 1.00±0.03ns | 9 | 0.562ns |
wt-4-C8166 | 1.02±0.02ns | 3 | 1.575ns | 1.03±0.03ns | 9 | 0.151ns |
wt-5-C8166 | 1.00±0.02ns | 3 | 1.443ns | 1.01±0.02ns | 9 | 0.062ns |
wt-6-C8166 | 1.01±0.01ns | 3 | 1.509ns | 1.04±0.02ns | 9 | 0.754ns |
mt-1-C8166 | 0.86±0.02ns | 1 | 0.620ns | 0.82±0.06ns | 8 | 1.215ns |
mt-2-C8166 | 0.78±0.06ns | 1 | 0.254ns | 0.89±0.03ns | 8 | 1.843ns |
mt-3-C8166 | 0.79±0.06ns | 1 | 0.117ns | 0.80±0.05ns | 8 | 2.164ns |
Mt-4-C8166 | 0.87±0.04ns | 1 | 0.995ns | 0.89±0.04ns | 8 | 1.016ns |
mt-5-C8166 | 0.77±0.07ns | 1 | 0.495ns | 0.86±0.04ns | 8 | 1.171ns |
mt-6-C8166 | 0.78±0.06ns | 1 | 0.277ns | 0.85±0.05ns | 8 | 1.188ns |
Fitness is relative to reference strain v13
Significance of fitness at day 110 was compared to the parental virus (wt/mt–d0) in an ANOVA containing all fitness samples measured during the experiment, p value was therefore Bonferroni-corrected for multiple comparisons
F statistic was determined in an ANOVA with repeated measures
d.f., degrees of freedom (number of fitness samples measured during experiment); n.s., not significant